Journal: PLoS ONE

Article Title: Specification of Region-Specific Neurons Including Forebrain Glutamatergic Neurons from Human Induced Pluripotent Stem Cells

doi: 10.1371/journal.pone.0011853

Figure Lengend Snippet: ( A & B ) Immunostaining for the forebrain functional markers TBR1 and MAP2 ( A ), and CTIP2 and VGLUT1 ( B ) on cells differentiated for 5 ( A ) or 6 ( B ) weeks from H9 hESC and TZ1 and YZ1 hiPSC lines. ( C ) Immunostaining for the dopaminergic neuron marker TH on cells differentiated from hESC/hiPSC-derived and FGF8/SHH-treated neural progenitors. ( D ) Immunostaining for the spinal motor neuronal marker HB9 (with βIII-tubulin as a neuronal control marker) on cells differentiated from hESC/hiPSC-derived and RA/SHH-treated neural progenitors. ( E ) Some cells were positive for S100β (an astrocyte marker) or Synapsin at two months after differentiation from H9 or TZ1 cells. Cell nuclei were counterstained with Hoechst 33342. Bar, 50 µm. ( F ) Percentage of cells immunostained positive for TBR1, HB9, and TH counted for A , C , and D , respectively.

Article Snippet: Mouse anti-PAX6 (final dilution 1∶5000), rat anti-HOXB4 (1∶20), mouse anti-MNR2 (HB9, 1∶50), and rabbit anti-βIII-tubulin (1∶5000) antibodies were from Developmental Studies Hybridoma Bank (Iowa City, IA).

Techniques: Immunostaining, Functional Assay, Marker, Derivative Assay

Journal: bioRxiv

Article Title: The effect of sonic hedgehog on motor neuron positioning in the spinal cord during chicken embryo development

doi: 10.1101/178491

Figure Lengend Snippet: A-H: Shh overexpression following pCAGGS-Shh and pCAGGS-GFP co-transfection at 84 h. DAPI nuclear staining (A, higher magnification of the ventral areas in E), GFP expression (B, higher magnification of the ventral areas in F, green), MNR2 expression (C, higher magnification of the ventral areas in G, red), and merged images (D, higher magnification of the ventral areas in H). I-P: Control group after pCAGGS-GFP transfection at 84 h. DAPI nuclear stain (I, higher magnification of the ventral areas in M), GFP expression (J, higher magnification of the ventral areas in N), MNR2 expression (K, higher magnification of the ventral areas in O, red), and the merged image (L, higher magnification of the ventral areas in P). mc, motor column, Arrows (→) indicate the areas of MNR2 expression. Scale bars, 100 μm in A, E, I, M for A-P, respectively.

Article Snippet: The primary antibodies were then applied overnight at 4 o C. The primary antibodies used in the present study were rabbit anti Map2 polyclonal antibody (Abcam, United Kingdom, 1:500 dilution), mouse anti chicken MNR2 monoclonal antibody (DSHB, USA; 1:100 dilution), mouse anti Pax3 monoclonal antibody (DSHB, USA; 1:100 dilution), mouse anti Pax7 monoclonal antibody (DSHB, USA; 1:100 dilution), and mouse anti BrdU monoclonal antibody (ZSGB-BIO, China; 1:100 dilution).

Techniques: Over Expression, Cotransfection, Staining, Expressing, Control, Transfection

Journal: eLife

Article Title: Transcriptional control of motor pool formation and motor circuit connectivity by the LIM-HD protein Isl2

doi: 10.7554/eLife.84596

Figure Lengend Snippet:

Article Snippet: Rabbit anti-GFP (Invitrogen), mouse anti-Isl2 (DSHB), and mouse anti-MNR2 (DSHB) antibodies were used.

Techniques: Sequencing, Recombinant, Plasmid Preparation, Labeling, Software